What is PyOTC and what does it?¶
PyOTC is a python optical tweezers calibration software package. Its aim is to provide means to precisely calibrate a trapped particle by analyzing the power spectral density of its thermal motion.
In particular, it provides means for a height-dependent calibration of a trapped bead. This calibration approach is especially interesting for experiments that are carried out close to the sample-chamber surface, where conventional PSD analysis is difficult or even impossible. It is also useful in cases where the size of the bead or the viscosity of the sample medium and/or the actual height of the bead is unknown.
The package provides the following features:
- calculation of power spectral density from time-dependent signals
- saving and loading of PSD measurments
- active and passive power spectral density analysis
- fitting of PSDs to different models
- consideration of low-pass filtering and aliasing of the signals
- height-dependent analysis of active PSD measurements
Height-dependent analysis of active PSD calibrations enables you to determine the
- particle size or
- viscosity of the medium,
- location of the sample surface and
- height-dependence of the trap stiffness and displacement sensitivity
PyOTC is written in Python 3.4 and was developed for the use with Jupyter.
